Biosynthesis of rubber: leaf-disc experiments. (Monthly Report No. 59, May 1980)

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Title: Biosynthesis of rubber: leaf-disc experiments. (Monthly Report No. 59, May 1980)
Creator: Bealing, F.J.
Contributor: Selamah Bidin
Contributor: Mohd. Norafendy bin Mohd. Nordin
Subject: Misc./Others
Subject: Biosynthesis
Subject: Plant propagation and growth
Date: 2012-11-6 |2013-02-04 |2001–1 |1980 | | | | | | |
Date: 1980
Description: Leaf-disc experiments have so far been performed in 0.005 M potassium hydrogen phthalate containing 0.001 M CaSO^, the medium used in earlier work on the glycolateserine pathway of isoprone formation in Hamamelis leaf discs (Jones and Kasmussen, 1 9 7 5 ) . CaSO^ promotes the uptake of serine into plant cells, but there was no evidence that it was of value in the present glycolate and glycine incubations. This was investigated by comparing the conversion of glycolate to rubber in discs which had been infiltrated either with phthalate:CaSO^ (pll 4 . 1 ), or with phthalate alone (also pH 4 . 1 ) . A further batch of discs was infiltrated with phthalate: CaS0,^ augmented with 0.01 M HPMS (2-pyridylhydr oxymethane sulphonic acid, adjusted to pll 4 . 1 with NaOIl) , at this concentration a widely-used inhibitor of the oxidation of glycolate to glyoxylate. All of the discs were from mature leaves from the same branch.

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Bealing, F.J.. Biosynthesis of rubber: leaf-disc experiments. (Monthly Report No. 59, May 1980). 2012-11-6 |2013-02-04 |2001–1 |1980 | | | | | | |.

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